New preprint: evaluating a laboratory workflow for sequencing Mycobacteria using long-read sequencing

In this preprint, we describe and evaluate a laboratory workflow for sequencing Mycobacterial samples using long-read sequencing (as exemplified by Oxford Nanopore Technologies). A key part of this manuscript is the development of a lab protocol that combines rapid, semi-automated DNA extraction from MGIT cultures with PCR-based whole-genome amplification.