New paper: detecting compensatory mutations in the RNAP of M. tuberculosis Philip Fowler, 5th February 20245th February 2024 In this paper, by examining testing the association between mutations known to be associate with resistance and other mutation in the M. tuberculosis RNA polymerase, Viki Brunner is able to identify 51 putative compensatory mutations. This is only possible thanks to the large CRyPTIC dataset containing of over 70,000 TB genomes. The paper is also the first use at scale of the growth in the positive control wells as measured by AMyGDA after two weeks of incubation and we are able to recapitulate the original in vitro result that mutations in RNAP that confer resistance are associated with a fitness cost (here assumed to be growth). This is the peer-reviewed version of an earlier preprint. Share this: Click to share on X (Opens in new window) X Click to share on Bluesky (Opens in new window) Bluesky Click to email a link to a friend (Opens in new window) Email Click to share on LinkedIn (Opens in new window) LinkedIn Click to share on Mastodon (Opens in new window) Mastodon Related antimicrobial resistance clinical microbiology publication tuberculosis
New preprint: a deep learning model that can read 96-well broth micro dilution plates 23rd February 202523rd February 2025 The CRyPTIC project used bespoke 96-well broth microdilution plates to measure the minimum inhibitory concentrations… Share this: Click to share on X (Opens in new window) X Click to share on Bluesky (Opens in new window) Bluesky Click to email a link to a friend (Opens in new window) Email Click to share on LinkedIn (Opens in new window) LinkedIn Click to share on Mastodon (Opens in new window) Mastodon Read More
publication New Publication: NRas slows the rate at which a model lipid bilayer phase separates 13th June 2014 Here we examine by computer simulation what effect adding a small cell-signalling protein does… Share this: Click to share on X (Opens in new window) X Click to share on Bluesky (Opens in new window) Bluesky Click to email a link to a friend (Opens in new window) Email Click to share on LinkedIn (Opens in new window) LinkedIn Click to share on Mastodon (Opens in new window) Mastodon Read More
antimicrobial resistance New publication: Automated detection of bacterial growth on 96-well plates for high-throughput drug susceptibility testing of M. tuberculosis 26th October 2018 In this Microbiology paper we show how a Python package, called the Automated Mycobacterial Detection Growth… Share this: Click to share on X (Opens in new window) X Click to share on Bluesky (Opens in new window) Bluesky Click to email a link to a friend (Opens in new window) Email Click to share on LinkedIn (Opens in new window) LinkedIn Click to share on Mastodon (Opens in new window) Mastodon Read More