New Publication: Flexible Gates Generate Occluded Intermediates in the Transport Cycle of LacY Philip Fowler, 8th November 2013 In this paper we examine how the lactose permease, LacY, changes its structure to shuttle molecules of lactose across a cell membrane. The change in conformation is modelled usinga biased computational method called dynamic importance sampling (DIMS) and the results compared to the results of some previously published double electron electron spin resonance (DEER) experiments. We conclude that LacY, as expected, does pass through an occluded intermediate and this is incompatible a simple rigid-body motion as implied by a “rocker-switch” mechanism. It is published in the Journal of Molecular Biology and is free to download (open access). Share this:TwitterBlueskyEmailLinkedInMastodon Related publication research
New preprint: validating antibiotic resistance prediction in our Myco pipeline 9th November 202413th January 2025 Over the last 18 months or so we’ve been designing, coding and testing a Mycobacterial… Share this:TwitterBlueskyEmailLinkedInMastodon Read More
antimicrobial resistance Successful NIHR grant 29th June 20185th August 2018 Last year I coordinated a bid to the NIHR for capital to improve our research… Share this:TwitterBlueskyEmailLinkedInMastodon Read More
antimicrobial resistance New preprint: predicting rifampicin resistance 16th August 202416th August 2024 In this preprint we train a series of machine learning models on protein mutations found… Share this:TwitterBlueskyEmailLinkedInMastodon Read More