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Fowler Lab

Predicting antibiotic resistance de novo

New preprint: ONT sequencing is comparable to Illumina sequencing for M. tuberculosis

Philip Fowler, 9th April 20269th April 2026

In this preprint, we compare the differences when Mycobacterial samples are sequenced with either long- or short-read sequencing and show that long-read sequencing (as exemplified by Oxford Nanopore Technologies) is now comparable to short-read sequencing (e.g. Illumina). As we are focussed on clinical microbiological outputs, we compare the ability of both approaches to resolve the species and, if M. tuberculosis, to predict to which antibiotics the infection will be susceptible.

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