New publication: Assessing Drug Susceptibility in Tuberculosis Philip Fowler, 28th September 201829th September 2018 A paper was published in the New England Journal of Medicine earlier this week by the CRyPTIC project, of which I am part, with help from the 100,000 genomes project. It demonstrates how whole genome sequencing can be used to accurately predict drug susceptibility for the four first-line anti-tubercular drugs (isoniazid, rifampicin, pyrazinamide and ethambutol) and, crucially, how the accuracy only degrades slowly as the local prevalence of resistance increases. All the 10,000 samples had qualitative drug susceptibility profiles gathered using the MGIT system which simply reports whether a sample is resistant or sensitive to a drug. CRyPTIC is currently collecting >30,000 samples using a 96-well plate containing 14 different drugs and therefore will be reporting quantitative data (i.e. minimum inhibitory concentrations). So, whilst this is a good first step, I expect the project to produce a wide range of even more exciting studies in the next few years. Since our ability to draw inferences from correlations between the genetic and phenotypic data depend critically on minimising the errors in the data, I have been heavily involved developing methods to allow us to quality control the measurements made in the different consortium laboratories. These are AMyGDA. This is software that automatically analyses photographs of the 96 well plates CRyPTIC is using. You can read more here. A paper is accepted and I’ll update this post when I have a link. BashTheBug. This is a Citizen Science project hosted on the Zooniverse where anyone can classify a few (or a thousand) images taken from the 96 well plates. These independent sets of measurements are then combined with those collected in the laboratories and a consensus is reached, hopefully minimising errors, improving signal to noise and reducing therefore the number of samples we need before we can start e.g. identifying new genes and genetic variants that can confer resistance to anti-TB drugs. In short, watch this space! Share this: Click to share on X (Opens in new window) X Click to share on Bluesky (Opens in new window) Bluesky Click to email a link to a friend (Opens in new window) Email Click to share on LinkedIn (Opens in new window) LinkedIn Click to share on Mastodon (Opens in new window) Mastodon Related antimicrobial resistance citizen science clinical microbiology publication tuberculosis
molecular dynamics New Publication: Alchembed 12th June 2015 In much of my research I’ve looked at how proteins embedded in cell membranes behave. An… Share this: Click to share on X (Opens in new window) X Click to share on Bluesky (Opens in new window) Bluesky Click to email a link to a friend (Opens in new window) Email Click to share on LinkedIn (Opens in new window) LinkedIn Click to share on Mastodon (Opens in new window) Mastodon Read More
antimicrobial resistance Updated preprint: predicting pyrazinamide resistance 21st November 20238th December 2023 This study was performed by Josh Carter back in 2019 and we uploaded a preprint… Share this: Click to share on X (Opens in new window) X Click to share on Bluesky (Opens in new window) Bluesky Click to email a link to a friend (Opens in new window) Email Click to share on LinkedIn (Opens in new window) LinkedIn Click to share on Mastodon (Opens in new window) Mastodon Read More
antimicrobial resistance New publication: Reconciling the potentially irreconcilable? Genotypic and phenotypic amoxicillin-clavulanate resistance in Escherichia coli. 30th March 202022nd August 2020 Clinical microbiology often assumes a sample is resistant or susceptible. Making such a classification relies… Share this: Click to share on X (Opens in new window) X Click to share on Bluesky (Opens in new window) Bluesky Click to email a link to a friend (Opens in new window) Email Click to share on LinkedIn (Opens in new window) LinkedIn Click to share on Mastodon (Opens in new window) Mastodon Read More